A fluorescence method for the determination of plasma susceptibility to lipid peroxidation.
Clin Biochem
; 32(3): 171-7, 1999 Apr.
Article
em En
| MEDLINE
| ID: mdl-10383076
ABSTRACT
OBJECTIVE:
We propose a fluorescence kinetics method for monitoring plasma susceptibility to peroxidation. DESIGN ANDMETHOD:
Plasmatic peroxidation was induced by CuSO4 (500 microM), and fluorescence was measured every 30 min. Kinetics were represented by a sigmoidal curve from which it was possible to calculate the latency time (lag-time) and the propagation velocity (slope) of plasma peroxidation.RESULTS:
The lag-time monitored by the fluorescence kinetics method corresponded to the formation of thiobarbituric acid reactive substances, and to progressive depletion of polyunsaturated fatty acids and alpha-tocopherol. The mechanism of reaction appeared to be dependent upon plasmatic hydroperoxides, and independent of oxygen radicals. Plasma storage is possible for at least two months at -80 degrees C, and reproducibility of the method is very good.CONCLUSIONS:
Fluorescence kinetics provide a highly comprehensive picture of plasma susceptibility to peroxidation in comparison with the conventional measurements of anti- and pro-oxidant ratios.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plasma
/
Espectrometria de Fluorescência
/
Peroxidação de Lipídeos
Limite:
Humans
Idioma:
En
Revista:
Clin Biochem
Ano de publicação:
1999
Tipo de documento:
Article
País de afiliação:
Itália