Characterization of cytokine expression in the rectal mucosa of ulcerative colitis: correlation with disease activity.
Am J Gastroenterol
; 94(9): 2441-6, 1999 Sep.
Article
em En
| MEDLINE
| ID: mdl-10484006
ABSTRACT
OBJECTIVE:
Mucosal inflammation in ulcerative colitis (UC) is presumed to be regulated by Th2-like cytokines. The aim of this study was to characterize local expression of various cytokines mRNA.METHODS:
Total RNA was extracted from rectal biopsy specimens in 61 patients with UC, 18 inflammatory controls, and 16 noninflammatory controls. Reverse-transcription polymerase chain reaction (RT-PCR) was used to determine mRNA expression of interleukin (IL)-2, interferon (IFN)-gamma, IL-4, IL-10, IL-13, and IL-15.RESULTS:
Expression of IL-10 was more frequent in UC (75.4%) than in noninflammatory controls (37.5%, p < 0.01). IL-4 was more frequently positive in UC (41%) than in inflammatory controls (5.6%, p < 0.01) and in noninflammatory controls (6.3%, p < 0.01). Positive expressions of IL-4 (66.7% vs 20.6%, p < 0.01) and IL-13 (63.0% vs 29.4%, p < 0.01) were higher in active UC than in inactive UC. The positive rate of IL-2, interferon (IFN)-gamma, and IL-15 expression showed no difference among the groups divided by clinical, endoscopic, and histological grade of inflammation.CONCLUSIONS:
These findings suggest that in active UC, IL-4 is pivotal, in combination with other Th2-like cytokines. In contrast, Th1-like cytokines and IL-15 bear no definite relation to local inflammation of UC.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Reto
/
Colite Ulcerativa
/
Interleucinas
/
Interferon gama
/
Mucosa Intestinal
Limite:
Adolescent
/
Adult
/
Aged
/
Child
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
Am J Gastroenterol
Ano de publicação:
1999
Tipo de documento:
Article
País de afiliação:
Japão