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Arterial injury increases expression of inflammatory adhesion molecules in the carotid arteries of apolipoprotein-E-deficient mice.
Manka, D R; Wiegman, P; Din, S; Sanders, J M; Green, S A; Gimple, L W; Ragosta, M; Powers, E R; Ley, K; Sarembock, I J.
Afiliação
  • Manka DR; Department of Biomedical Engineering, University of Virginia, Health Sciences Centers, Charlottesville, VA 22908, USA.
J Vasc Res ; 36(5): 372-8, 1999.
Article em En | MEDLINE | ID: mdl-10559677
ABSTRACT
Recent studies demonstrate increased cellular adhesion molecule expression by neointimal endothelium overlying primary and restenotic atherosclerotic plaque. In this study, we developed an atherosclerotic mouse model of arterial injury and characterized adhesion molecule expression after injury. Sixteen apolipoprotein-E-(ApoE)-deficient mice fed a Western-type diet for 4 weeks underwent carotid artery wire denudation at week 2. For each segment, the extent of neointima formation and medial thickening, or adhesion molecule expression, were scored separately on a scale from 0 (no plaque/thickening or expression) to 3 (extensive plaque/thickening or expression) using Movat staining (n = 3) or immunohistochemical analysis (n = 13). Histology revealed significant medial thickening (1.8 +/- 0.9 vs. 0.3 +/- 0.5, p < 0. 001) versus controls and pronounced staining for monocytes/macrophages in the wall of injured vessels. Immunohistochemical analysis showed more robust expression of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) on the luminal surface of injured arteries versus controls (2.2 +/- 0.6 vs. 1.4 +/- 0.7, p < 0.01, and 2.5 +/- 0.5 vs. 1.2 +/- 0.6, p < 0.001, respectively). Injury increased adventitial ICAM-1 expression (2.6 +/- 0.5 vs. 1.6 +/- 0.5, p < 0.002) and medial VCAM-1 expression (2.2 +/- 0.6 vs. 1.2 +/- 0. 7, p < 0.004). Thus, carotid injury results in significant medial thickening and increases adhesion molecule expression beyond that induced in ApoE-deficient mice fed a Western diet alone. The observation of macrophage infiltration into the media at sites of increased ICAM-1 and VCAM-1 expression suggests that these molecules may mediate monocyte/macrophage trafficking into the wall of injured arteries.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Apolipoproteínas E / Arteriosclerose / Artérias Carótidas / Molécula 1 de Adesão Intercelular / Molécula 1 de Adesão de Célula Vascular / Lesões das Artérias Carótidas Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: J Vasc Res Assunto da revista: ANGIOLOGIA Ano de publicação: 1999 Tipo de documento: Article País de afiliação: Estados Unidos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Apolipoproteínas E / Arteriosclerose / Artérias Carótidas / Molécula 1 de Adesão Intercelular / Molécula 1 de Adesão de Célula Vascular / Lesões das Artérias Carótidas Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: J Vasc Res Assunto da revista: ANGIOLOGIA Ano de publicação: 1999 Tipo de documento: Article País de afiliação: Estados Unidos
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