SLG1 plays a role during G1 in the decision to enter or exit the cell cycle.
Mol Gen Genet
; 262(6): 1147-56, 2000 Jan.
Article
em En
| MEDLINE
| ID: mdl-10660075
ABSTRACT
Saccharomyces cerevisiae cells decide to divide during G1. If nutrients are abundant, cells pass through START and coordinately undergo DNA replication, bud emergence, and spindle pole body duplication. Phenotypic analysis of the slg1delta mutant revealed that this mutation uncouples post-START events. At the nonpermissive temperature, slg1delta cells that have undergone bud emergence but not DNA replication or SPB duplication accumulate. Furthermore, while wild-type cells arrest in GO when starved, the slg1delta mutant fails to arrest at this point; instead, cells with small buds accumulate. The slg1delta mutation displayed genetic interactions with cdc34, which encodes a regulator of exit from G1. This is consistent with a role of SLG1 in G1 regulation. Epitope-tagged Slg1p cofractionated with the plasma membrane, suggesting that Slglp may function by integrating external cues and relaying them to the interior of the cell. We propose that SLG1 plays a regulatory role in bud emergence or stationary phase.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
/
Glicoproteínas de Membrana
/
Fase G1
/
Proteínas de Saccharomyces cerevisiae
Idioma:
En
Revista:
Mol Gen Genet
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Estados Unidos