Quantitative analysis of constitutive and inducible CYPs mRNA expression in the HepG2 cell line using reverse transcription-competitive PCR.
Biochem Biophys Res Commun
; 267(3): 756-60, 2000 Jan 27.
Article
em En
| MEDLINE
| ID: mdl-10673364
ABSTRACT
Drug interactions which affect drug metabolism are of clinical importance. It is, however, difficult to estimate drug interactions in human from results obtained in animal experiments. In our previous study, we demonstrated that a combination of the HepG2 cell line and semiquantitative reverse transcription-PCR (RT-PCR) could be used to evaluate the degree of CYP3A mRNA induction by various drugs. Using an RT-competitive PCR (RT-cPCR) with beta-actin as the standard in this study, the constitutive and rifampicin (RFP)-induced expression of CYP3A4, CYP2C9, CYP2E1, and CYP1A2 mRNA in the HepG2 cells could be quantitatively and reproducibly determined. 120 h-treatment of HepG2 cells with 50 micromol/l RFP induced maximally 8.4- and 6.0-fold the expression of CYP3A4 and CYP2C9 mRNA, respectively, in comparison with untreated cells. On the other hand, mRNA level in CYP2E1 and CYP1A2 was not significantly changed by 50 micromol/l RFP after 24 to 120 h. To our knowledge, we report for the first time quantitative profiles of CYPs mRNA in HepG2 cells. This study demonstrates the efficiency of a combination of HepG2 cells and RT-cPCR in the evaluation of CYPs mRNA-induction by drugs.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Esteroide Hidroxilases
/
Transcrição Gênica
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Hidrocarboneto de Aril Hidroxilases
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Regulação Enzimológica da Expressão Gênica
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Citocromo P-450 CYP1A2
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Citocromo P-450 CYP2E1
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Sistema Enzimático do Citocromo P-450
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Esteroide 16-alfa-Hidroxilase
/
Oxigenases de Função Mista
Limite:
Humans
Idioma:
En
Revista:
Biochem Biophys Res Commun
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Japão