Evidence that 3'-phosphorylated polyphosphoinositides are generated at the nuclear surface: use of immunostaining technique with monoclonal antibodies specific for PI 3,4-P(2).
FEBS Lett
; 473(2): 222-6, 2000 May 12.
Article
em En
| MEDLINE
| ID: mdl-10812079
ABSTRACT
Phosphatidylinositol (PI) 3,4-P(2) is a phosphoinositide that has been shown to be important for signal transduction in growth factor stimulation. We have produced monoclonal antibodies specific for PI 3,4-P(2), which were able to detect PI 3,4-P(2) generated in 293T cells treated with H(2)O(2), or in MKN45/BD110 cells expressing activated PI 3-kinase in immunostaining. Prolonged treatment with 0.05% Tween 20 resulted in detection of staining not only at the plasma membrane, but also at the nuclear surface, indicating that 3'-phosphorylated phosphoinositides can be generated and function in the nucleus.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fosfatos de Fosfatidilinositol
/
Membrana Nuclear
Limite:
Animals
/
Humans
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Japão