Cellular proteins bind to the 3' and 5' untranslated regions of dengue 2 virus genome.
Malays J Pathol
; 20(1): 11-7, 1998 Jun.
Article
em En
| MEDLINE
| ID: mdl-10879258
ABSTRACT
In vitro generated cloned full length dengue 2 virus untranslated regions (UTRs) were used in RNA gel mobility shift assays to examine cellular factors binding to the virus genomes. Cellular factors in lysates of Vero (monkey) and C6/36 (mosquito) cells bound specifically and non-specifically to the dengue 2 virus 3' UTR. Non-specific interaction with the 5' UTR, resulting in formation of at least 4 band shift complexes was noted with lysate of the C6/36 cells only. Pre-treating the cell lysates with proteinase K affected binding of cellular factors to the dengue 2 virus UTRs, suggesting that the cellular factors were proteins. These findings suggest that cellular proteins could interact with specific sites on the dengue virus genomes.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Contexto em Saúde:
3_ND
Problema de saúde:
3_dengue
Assunto principal:
RNA Viral
/
Proteínas de Ligação a RNA
/
Genoma Viral
/
Regiões 3' não Traduzidas
/
Regiões 5' não Traduzidas
/
Vírus da Dengue
Limite:
Animals
Idioma:
En
Revista:
Malays J Pathol
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Malásia