A first-tier rapid assay for the serodiagnosis of Borrelia burgdorferi infection.
Arch Intern Med
; 161(16): 2015-20, 2001 Sep 10.
Article
em En
| MEDLINE
| ID: mdl-11525704
ABSTRACT
BACKGROUND:
The present recommendation for the serologic diagnosis of Lyme disease is a 2-tier process in which a serum sample with a positive or equivocal result by an enzyme-linked immunosorbent assay (ELISA) or immunofluorescent assay is then followed by supplemental testing by Western blot. Our laboratory has developed recombinant chimeric proteins composed of key Borrelia epitopes. These novel antigens are consistent and are easily standardized.METHODS:
We adapted these recombinant proteins into a new immunochromatographic format that can be used as a highly sensitive and specific first-tier assay that can be used to replace the ELISA or immunofluorescent assay.RESULTS:
This rapid test was equally sensitive (P>.05) and more specific (P<.05) than a frequently used commercial whole cell ELISA. The overall clinical accuracy achieved on agreement studies among 3 Lyme research laboratories on clinically defined serum panels was shown to be statistically equivalent to the commercial ELISA. The assay can detect anti-Borrelia burgdorferi antibodies in either serum or whole blood.CONCLUSION:
This sensitive and specific rapid assay, which is suited for the physician's office, streamlines the 2-tier system by allowing the physician to determine if a Western blot is necessary at the time of the initial office visit.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Infecções por Borrelia
/
Proteínas Recombinantes de Fusão
/
Grupo Borrelia Burgdorferi
/
Cromatografia
/
Anticorpos Antibacterianos
/
Epitopos
Tipo de estudo:
Diagnostic_studies
/
Guideline
Limite:
Humans
Idioma:
En
Revista:
Arch Intern Med
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Estados Unidos