In vitro function of S rnases in Lycopersicon peruvianum.
Mol Cells
; 12(3): 329-35, 2001 Dec 31.
Article
em En
| MEDLINE
| ID: mdl-11804331
ABSTRACT
S RNases are products of the S locus that are expressed in the stylar tissue of Lycopersicon peruvianum with the gametophytic self-incompatibility (SI) system. Two S RNases (S12 and Sa) with RNase activity from the S12Sa genotype of L. peruvianum were purified using gel filtration and cation-exchange chromatography. The molecular masses of the two RNases, S12 and Sa, were 21 and 23.1 kDa, respectively. The specific activities of S12 and Sa for torula yeast rRNA as a substrate were 8,500 and 6,000 units/ml, respectively. Of various reagents tested for RNase activities, ZnSO4 and CuSO4 were found to remarkably reduce its activity. The growth of S12Sa pollen was inhibited when it was cultured in a pollen germination medium that contained the purified S12 RNase. The result suggested that the S RNase was either a probable inhibitor of pollen growth or controlled pollen growth. Additionally, 512Sa pollens germinated well in vitro in a germination medium that contained S12 RNase in the presence of ZnSO4 and CuSO4. Our finding suggests that the treatment of S RNase with its inhibitor destroys the SI ability on an in vitro self-pollen growth test.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ribonucleases
/
Solanaceae
/
Isoenzimas
Idioma:
En
Revista:
Mol Cells
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2001
Tipo de documento:
Article