Malondialdehyde production in Jurkat T cells subjected to oxidative stress.
Nutrition
; 19(6): 545-8, 2003 Jun.
Article
em En
| MEDLINE
| ID: mdl-12781857
ABSTRACT
OBJECTIVE:
We investigated the relation between membrane lipid peroxidation, as evaluated by malondialdehyde (MDA), and oxidative stimuli in the Jurkat T-cell line and designed a cellular model to assess the antioxidant potential of compounds.METHODS:
Jurkat T cells were subjected to different concentrations of Fe(2+) ions (from 25 to 150 micromol/L) or H(2)O(2) (from 0.1 to 5 mmol/L), and MDA was determined after separation in high-performance liquid chromatography of the adduct with thiobarbituric acid. MDA production also was investigated in cells supplemented with epigallocatechin gallate and genistein and subjected to Fe(2+) oxidative treatment.RESULTS:
MDA production increased with the concentration of Fe(2+), whereas H(2)O(2) had no effect at any concentration. Oxidative stress for 15 min or 2 h produced similar MDA levels. The supplementation of epigallocatechin gallate partly prevented MDA production (about 40%, P < 0.05), whereas genistein exerted no preventive effect on lipid peroxidation.CONCLUSION:
We propose this cellular model, consisting of Jurkat T cells subjected to 100 micromol/L of Fe(2+) for 15 min, to study the protective effect of antioxidant supplementation against membrane lipid peroxidation.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Linfócitos T
/
Catequina
/
Estresse Oxidativo
/
Malondialdeído
Limite:
Humans
Idioma:
En
Revista:
Nutrition
Assunto da revista:
CIENCIAS DA NUTRICAO
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Itália