Role of mammalian Rad54 in telomere length maintenance.
Mol Cell Biol
; 23(16): 5572-80, 2003 Aug.
Article
em En
| MEDLINE
| ID: mdl-12897131
ABSTRACT
The homologous recombination (HR) DNA repair pathway participates in telomere length maintenance in yeast but its putative role at mammalian telomeres is unknown. Mammalian Rad54 is part of the HR machinery, and Rad54-deficient mice show a reduced HR capability. Here, we show that Rad54-deficient mice also show significantly shorter telomeres than wild-type controls, indicating that Rad54 activity plays an essential role in telomere length maintenance in mammals. Rad54 deficiency also resulted in an increased frequency of end-to-end chromosome fusions involving telomeres compared to the controls, suggesting a putative role of Rad54 in telomere capping. Finally, the study of mice doubly deficient for Rad54 and DNA-PKcs showed that telomere fusions due to DNA-PKcs deficiency were not rescued in the absence of Rad54, suggesting that they are not mediated by Rad54 activity.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Recombinação Genética
/
Telômero
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Proteínas de Saccharomyces cerevisiae
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Reparo do DNA
Limite:
Animals
Idioma:
En
Revista:
Mol Cell Biol
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Espanha