Theophylline and prostaglandin E2 on duodenal bicarbonate secretion: role for 5'-cyclic adenosine monophosphate.
Gastroenterology
; 103(1): 153-9, 1992 Jul.
Article
em En
| MEDLINE
| ID: mdl-1319368
ABSTRACT
Cyclic adenosine monophosphate (cAMP) has been implicated as an intracellular "second" messenger in duodenal mucosal bicarbonate secretion in animals. The purpose of this study was to determine whether cAMP may mediate duodenal mucosal bicarbonate secretion in humans. In healthy volunteers, a 4-cm segment of proximal duodenum was isolated from gastric and pancreaticobiliary secretions. Either the phosphodiesterase inhibitor theophylline, prostaglandin (PG) E2, or a combination thereof was administered topically to the isolated duodenal mucosa. Theophylline (10(-2) mol/L) and PGE2 (10(-5)-10(-4) mol/L) each significantly increased bicarbonate secretion and transmucosal potential difference. Moreover, when theophylline and PGE2 were administered in combination, the duodenal bicarbonate output was additive compared to either agent alone. When theophylline was infused with increasing doses of PGE2, the dose-response curve was shifted to the left. Furthermore, increases in bicarbonate secretion and transmucosal potential difference were correlated significantly. These results suggest that cAMP may act as an intracellular mediator of human duodenal mucosal bicarbonate secretion.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Teofilina
/
Bicarbonatos
/
Dinoprostona
/
AMP Cíclico
/
Duodeno
Limite:
Adult
/
Humans
/
Male
Idioma:
En
Revista:
Gastroenterology
Ano de publicação:
1992
Tipo de documento:
Article