Genomic analysis of a recently identified virus (SEN virus) and genotypes D and H by polymerase chain reaction.
Antiviral Res
; 60(1): 27-33, 2003 Sep.
Article
em En
| MEDLINE
| ID: mdl-14516918
ABSTRACT
BACKGROUND/AIMS:
SEN virus (SENV) was discovered in 1999 as a DNA virus with hepatotropic properties. Nine genotypes (A-I) have been identified with genotypes D and H being more prevalent in cases of chronic hepatitis. Attempts to determine whether SENV causes liver disease have been hampered by limited diagnostic testing.METHODS:
In the present study, we developed two PCR based assays; a general SENV screening and genotype-specific assay.RESULTS:
By screening PCR, the specificity for all SENV genotypes and SENV-related sequences was 20/20 (100%) with confirmation of the results being provided by genomic sequencing. With the genotype-specific PCR, specificities for SENV-D and SENV-H were 7/7 (100%) and 7/11 (64%), respectively. All screening PCR products were cloned and sequenced. The results of sequencing showed high genetic diversity in representative SENV genotypes. Five of twenty patients (25%) had mixed infections with several SENV genotypes.CONCLUSIONS:
The screening PCR was useful for identifying cases of SENV infection. However, because of high genetic divergence and mixed co-infection, it was difficult to establish a specific method for genotype distinction. Hence, sequencing is still required for further investigations of SENV as a potential cause of liver disease.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Reação em Cadeia da Polimerase
/
Infecções por Circoviridae
/
Circoviridae
/
Hepatite Crônica
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
/
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Antiviral Res
Ano de publicação:
2003
Tipo de documento:
Article