[Association of receptor-mediated endocytosis and autophagy with apoptosis].
Di Yi Jun Yi Da Xue Xue Bao
; 23(10): 1025-7, 2003 Oct.
Article
em Zh
| MEDLINE
| ID: mdl-14559683
ABSTRACT
OBJECTIVE:
To evaluate the relationship between receptor-mediated endocytosis, autophagosome formation and apoptosis by observing the morphological changes of mouse peritoneal macrophages in the course of receptor-mediated endocytotic activities, autophagy and apoptosis.METHODS:
Mouse peritoneal macrophages were incubated with horseradish peroxidase-labeled concanavalin A (ConA-HRP), and morphological examinations were performed at different time points after the incubation.RESULTS:
After the incubation of the macrophages with ConA-HRP, ConA-HRP was observed to enter the vesicles by way of receptor-mediated endocytosis. Three kinds of endosomes were observed, namely vesicles, tubes and double-membrane sheets. The double-membrane sheets enveloped a portion of the cytoplasm and organelles, thus giving rise to vesicles, or the autophagosomes, which later fused with lysosome, followed by the apoptosis of the macrophages.CONCLUSION:
Receptor-mediated endocytosis of ConA-HRP is associated with autophagy and apoptosis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Autofagia
/
Apoptose
/
Macrófagos Peritoneais
/
Receptores de Superfície Celular
/
Endocitose
Tipo de estudo:
Risk_factors_studies
Limite:
Animals
Idioma:
Zh
Revista:
Di Yi Jun Yi Da Xue Xue Bao
Assunto da revista:
MEDICINA
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
China