[Primary culture of human periodontal ligament fibroblasts by explants with enzymatic digestion].
Zhonghua Yi Xue Za Zhi
; 84(8): 656-8, 2004 Apr 17.
Article
em Zh
| MEDLINE
| ID: mdl-15130307
ABSTRACT
OBJECTIVE:
To increase the success rate of primary culture of human periodontal ligament fibroblasts (HPLF), and to establish an experimental model for studying HPLF in vitro.METHODS:
The primary cells were isolated from human periodontal ligament by explants with enzymatic digestion method. Morphological analysis and immunocytochemical staining were used to characterize the cell lineage, and growth curve assay to evaluate the biological features of HPLF.RESULTS:
The success rate of primary culture of HPLF was 77.8%. Cultured cells were spindle-shaped, and had a positive reaction to antibodies against vimentin, and a negative reaction to antibodies against keratin. Their morphological and biological characteristics were similar to those of typical HPLF. Growth of HPLF obtained by this method was satisfactory.CONCLUSION:
The success rate of primary culture of HPLF is significantly increased by explants combined with enzymatic digestion. The method is simple and feasible.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ligamento Periodontal
/
Técnicas de Cultura de Células
/
Fibroblastos
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
Zh
Revista:
Zhonghua Yi Xue Za Zhi
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
China