Triple helix formation with Drosophila satellite repeats. Unexpected stabilization by copper ions.
Biochemistry
; 43(35): 11196-205, 2004 Sep 07.
Article
em En
| MEDLINE
| ID: mdl-15366929
ABSTRACT
The Drosophila melanogaster (AAGAGAG)(n) satellite repeat represents up to 1.5% of the entire fly genome and may adopt non-B DNA structures such as pyrimidine triple helices. UV melting and electrophoretic mobility shift assay experiments were used to monitor the stability of intermolecular triple helices as a function of size, pH, and backbone or base modification. Three to four repeats of the heptanucleotide motif were sufficient to allow the formation of a stable complex, especially when modified TFOs were used. Unexpectedly, low concentrations (40-100 microM) of Cu(2+) were found to favor strongly pyrimidine triplex formation under near-physiological conditions. In contrast, a much higher magnesium concentration was required to stabilize these triplexes significantly, suggesting that copper may be an essential stabilizing factor for pyrimidine triplexes.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
/
DNA Satélite
/
Sequências Repetitivas de Ácido Nucleico
/
Cobre
/
Drosophila melanogaster
/
Conformação de Ácido Nucleico
Limite:
Animals
Idioma:
En
Revista:
Biochemistry
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
França