HMW1 is required for stability and localization of HMW2 to the attachment organelle of Mycoplasma pneumoniae.
J Bacteriol
; 186(24): 8221-8, 2004 Dec.
Article
em En
| MEDLINE
| ID: mdl-15576770
ABSTRACT
The cytoskeletal proteins HMW1 and HMW2 are components of the terminal organelle of the cell wall-less bacterium Mycoplasma pneumoniae. HMW1 is required for a tapered, filamentous morphology but exhibits accelerated turnover in the absence of HMW2. Here, we report that a reciprocal dependency exists between HMW1 and HMW2, with HMW2 subject to accelerated turnover with the loss of HMW1. Furthermore, the instability of HMW2 correlated with its failure to localize to the attachment organelle. The C-terminal domain of HMW1 is essential for both function and its accelerated turnover in the absence of HMW2. We constructed HMW1 deletion derivatives lacking portions of this domain and examined each for stability and function. The C-terminal 41 residues were particularly important for proper localization and function in cell morphology and P1 localization, but the entire C-terminal domain was required to stabilize HMW2. The significance of these findings in the context of attachment organelle assembly is considered.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
Aderência Bacteriana
/
Organelas
/
Regulação Bacteriana da Expressão Gênica
/
Adesinas Bacterianas
/
Proteínas de Membrana
/
Mycoplasma pneumoniae
Limite:
Humans
Idioma:
En
Revista:
J Bacteriol
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Estados Unidos