Expression and assay of HECT domain ligases.
Methods Enzymol
; 398: 112-25, 2005.
Article
em En
| MEDLINE
| ID: mdl-16275324
ABSTRACT
HECT domain ubiquitin ligases (HECT E3s), typified by human E6AP and yeast Rsp5p, are unique among the several classes of known ubiquitin ligases in that they participate directly in the chemistry of substrate ubiquitination reactions. This chapter discusses strategies for the expression of active HECT E3s and the assays that are available for analyzing E2 interaction, ubiquitin-thioester formation, and substrate ubiquitination.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ubiquitina-Proteína Ligases
Limite:
Humans
Idioma:
En
Revista:
Methods Enzymol
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Estados Unidos