Propane 2-nitronate is more rapidly denitrified and is more genotoxic than 2-nitropropane in cultured rat hepatoma cells.
Toxicol Lett
; 61(2-3): 149-57, 1992 Jul.
Article
em En
| MEDLINE
| ID: mdl-1641862
ABSTRACT
We have investigated the importance of nitronate formation from 2-nitropropane (2-NP) for the oxidative metabolism and the genotoxicity of 2-NP in 2sFou rat hepatoma cells. Treatment of the cells with 2-NP for up to 3 h resulted in the time-dependent appearance of nitrite in the culture medium and in a weak induction of DNA repair synthesis. Both nitrite formation and repair induction were markedly enhanced in cells exposed to the anionic form of 2-NP, propane 2-nitronate. These observations suggest that propane 2-nitronate, rather than 2-NP itself, is oxidized by the liver cells to yield a DNA-damaging product. The results also indicate that the nitro/nitronate equilibration in intact liver cells is slow, suggesting that nitronate formation represents the rate-limiting step in the metabolic activation of 2-NP.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Propano
/
Neoplasias Hepáticas Experimentais
/
Nitroparafinas
Limite:
Animals
Idioma:
En
Revista:
Toxicol Lett
Ano de publicação:
1992
Tipo de documento:
Article
País de afiliação:
Alemanha