PCR detection of lizard malaria parasites: prevalence of Plasmodium infections with low-level parasitemia differs by site and season.
J Parasitol
; 91(6): 1509-11, 2005 Dec.
Article
em En
| MEDLINE
| ID: mdl-16539045
ABSTRACT
Plasmodium-specific polymerase chain reaction (PCR) primers allowed detection of infections with very low-level parasitemia for 3 species of malaria parasites infecting Anolis lizards at 2 Caribbean sites, Puerto Rico and Saba, Netherlands Antilles. A verification study, using a single-tube nested PCR to eliminate contamination, showed that infections as low as 1 parasite per millions of erythrocytes could be detected by amplifying a 673 bp fragment of the cytochrome b gene. Very low-level parasitemia infections, subpatent under the microscope, were common in A. sabanus on Saba sites, with no significant seasonal difference (31% of infections appearing uninfected by microscopic examination in summer were found infected by PCR, 38% in winter). At the Puerto Rico site, the subpatent infections were also common in A. gundlachi, but were more prevalent in winter (53%) than in summer (17%). A similar high frequency of subpatent infections is known from studies on human and bird malaria, but a previous PCR-based study on a temperate lizard malaria system found few such low-level infections. Differences in the prevalence of subpatent infections by site and season suggest transmission biology may select for distinct life history strategies by the parasite.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Contexto em Saúde:
2_ODS3
/
3_ND
Problema de saúde:
2_enfermedades_transmissibles
/
3_malaria
/
3_zoonosis
Assunto principal:
Plasmodium
/
Reação em Cadeia da Polimerase
/
Parasitemia
/
Lagartos
/
Malária
Tipo de estudo:
Diagnostic_studies
/
Prevalence_studies
/
Risk_factors_studies
Limite:
Animals
País/Região como assunto:
Antillas holandesas
/
Caribe
/
Caribe ingles
/
Puerto rico
Idioma:
En
Revista:
J Parasitol
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Estados Unidos