Structure of the exon junction core complex with a trapped DEAD-box ATPase bound to RNA.
Science
; 313(5795): 1968-72, 2006 Sep 29.
Article
em En
| MEDLINE
| ID: mdl-16931718
ABSTRACT
In higher eukaryotes, a multiprotein exon junction complex is deposited on spliced messenger RNAs. The complex is organized around a stable core, which serves as a binding platform for numerous factors that influence messenger RNA function. Here, we present the crystal structure of a tetrameric exon junction core complex containing the DEAD-box adenosine triphosphatase (ATPase) eukaryotic initiation factor 4AIII (eIF4AIII) bound to an ATP analog, MAGOH, Y14, a fragment of MLN51, and a polyuracil mRNA mimic. eIF4AIII interacts with the phosphate-ribose backbone of six consecutive nucleotides and prevents part of the bound RNA from being double stranded. The MAGOH and Y14 subunits lock eIF4AIII in a prehydrolysis state, and activation of the ATPase probably requires only modest conformational changes in eIF4AIII motif I.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Poli U
/
RNA Mensageiro
/
Proteínas Nucleares
/
Éxons
/
Proteínas de Ligação a RNA
/
Fator de Iniciação 4A em Eucariotos
/
Proteínas de Neoplasias
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Science
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Dinamarca