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A uniaxial bioMEMS device for quantitative force-displacement measurements.
Serrell, David B; Oreskovic, Tammy L; Slifka, Andrew J; Mahajan, Roop L; Finch, Dudley S.
Afiliação
  • Serrell DB; National Institute of Standards and Technology, Boulder, CO, USA. serrell@colorado.edu
Biomed Microdevices ; 9(2): 267-75, 2007 Apr.
Article em En | MEDLINE | ID: mdl-17187300
There is a need for experimental techniques that allow the simultaneous imaging of cellular cystoskeletal components with quantitative force measurements on single cells. A bioMEMS device has been developed for the application of strain to a single cell while simultaneously quantifying its force response. The prototype device presented here allows the mechanical study of a single, adherent cell in vitro. The device works in a fashion similar to a displacement-controlled uniaxial tensile machine. The device is calibrated using an AFM cantilever and shows excellent agreement with the calculated spring constant. The device is demonstrated on a single fibroblast. The force response of the cell is seen to be linear until the onset of de-adhesion with the de-adhesion from the cell platform occurring at a force of approximately 1500 nN.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Transdutores / Fibroblastos / Micromanipulação Limite: Animals Idioma: En Revista: Biomed Microdevices Assunto da revista: ENGENHARIA BIOMEDICA Ano de publicação: 2007 Tipo de documento: Article País de afiliação: Estados Unidos
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Transdutores / Fibroblastos / Micromanipulação Limite: Animals Idioma: En Revista: Biomed Microdevices Assunto da revista: ENGENHARIA BIOMEDICA Ano de publicação: 2007 Tipo de documento: Article País de afiliação: Estados Unidos
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