Correlation of immunoreactivity and polymer formation to DTPA modification of a monoclonal antibody.
Int J Rad Appl Instrum B
; 18(8): 859-64, 1991.
Article
em En
| MEDLINE
| ID: mdl-1800461
ABSTRACT
The protocol used for coupling of monoclonal antibodies with mixed anhydride of DTPA for subsequent radiolabeling with indium-111 affects the integrity of the immunoreactivity of the antibody preparations. To analyze the effect of minor methodological variations on coupling characteristics, a two-step addition of DTPA to antimyosin antibody with gentle mixing was compared to a single addition with vigorous stirring. The molar ratios of DTPA to antibody were also varied. The polymer formation was assessed by SDS-PAGE and immunoreactivity was assessed by solid phase radioimmunoassay using human heart myosin as the antigen. The immunoreactivity was significantly decreased in the two-step, gentle-mixing method where polymer formation was evident. The one-step, vigorous-stirring method of DTPA incorporation produced no polymerization and no loss of immunoreactivity.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ácido Pentético
/
Anticorpos Monoclonais
Limite:
Humans
Idioma:
En
Revista:
Int J Rad Appl Instrum B
Assunto da revista:
BIOLOGIA
/
MEDICINA NUCLEAR
Ano de publicação:
1991
Tipo de documento:
Article