LINE-like retrotransposition in Saccharomyces cerevisiae.
Genetics
; 181(1): 301-11, 2009 Jan.
Article
em En
| MEDLINE
| ID: mdl-18957700
ABSTRACT
Over one-third of human genome sequence is a product of non-LTR retrotransposition. The retrotransposon that currently drives this process in humans is the highly abundant LINE-1 (L1) element. Despite the ubiquitous nature of L1's in mammals, we still lack a complete mechanistic understanding of the L1 replication cycle and how it is regulated. To generate a genetically amenable model for non-LTR retrotransposition, we have reengineered the Zorro3 retrotransposon, an L1 homolog from Candida albicans, for use in the budding yeast Saccharomyces cerevisiae. We found that S. cerevisiae, which has no endogenous L1 homologs or remnants, can still support Zorro3 retrotransposition. Analysis of Zorro3 mutants and insertion structures suggest that this is authentic L1-like retrotransposition with remarkable resemblance to mammalian L1-mediated events. This suggests that S. cerevisiae has unexpectedly retained the basal host machinery required for L1 retrotransposition. This model will also serve as a powerful system to study the cell biology of L1 elements and for the genetic identification and characterization of cellular factors involved in L1 retrotransposition.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
/
Elementos de DNA Transponíveis
/
Elementos Nucleotídeos Longos e Dispersos
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Genetics
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Estados Unidos