The time-course analysis of gene expression during wound healing in mouse skin.
Leg Med (Tokyo)
; 11(2): 70-5, 2009 Mar.
Article
em En
| MEDLINE
| ID: mdl-18974019
ABSTRACT
RNA analysis has been applied to forensic work to determine wound age. We investigated mRNA expression using quantitative RT-PCR of ten genes, including c-fos, fosB, mitogen-activated protein kinase phosphatase-1 (MKP-1), CD14, chemokine (C-C motif) ligand 9 (CCL9), placenta growth factor (PlGF), mast cell protease-5 (MCP-5), growth arrest specific 5 (Gas5), beta-2 microglobulin (B2M) and major urinary protein-1 (MUP-1), in terms of repair response in adult mice. The expression level of c-fos, fosB and MKP-1 transcripts increased drastically, peaked within 1h, and that of the CD14 and CCL9 transcripts peaked from 12 to 24h. An increase in PlGF and MCP-5 mRNA appeared on about day 5. Gas5, B2M and MUP-1 transcripts showed no significant change. Each gene had differentially expressional patterns with time-course. Our result implied that the observation of the 7 genes in wounded skin could serve to aid in the accurate diagnosis of wound age.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pele
/
Cicatrização
/
RNA Mensageiro
/
Expressão Gênica
Limite:
Animals
Idioma:
En
Revista:
Leg Med (Tokyo)
Assunto da revista:
JURISPRUDENCIA
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Japão