Identification and antifungal assay of a wheat beta-1,3-glucanase.
Biotechnol Lett
; 31(7): 1005-10, 2009 Jul.
Article
em En
| MEDLINE
| ID: mdl-19267236
ABSTRACT
A wheat beta-1,3-glucanase gene (TaGluD) was identified as a fungal defense candidate. Its transcript induction was more than 60-fold higher in a resistant wheat line, Shannong0431, than in a susceptible wheat line, Wenmai6, after infection with Rhizoctonia cerealis. The TaGluD protein was overexpressed as inclusion bodies in Escherichia coli. After refolding and purification, TaGluD with 1 unit of beta-1,3-glucanase showed antifungal activity in vitro against Rhizoctonia solani, R. cerealis, Phytophthora capsici and Alternaria longipes with inhibition rates of 42%, 43%, 32% and 30%, respectively. Thus TaGluD may be useful for enhancing fungal resistance in several crop species.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Contexto em Saúde:
3_ND
Problema de saúde:
3_neglected_diseases
/
3_zoonosis
Assunto principal:
Phytophthora
/
Rhizoctonia
/
Triticum
/
Glucana 1,3-beta-Glucosidase
/
Alternaria
/
Antifúngicos
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Biotechnol Lett
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
China