Measurement of proteoglycan mRNA in articular cartilage--preliminary investigations.
J Rheumatol Suppl
; 27: 55-7, 1991 Feb.
Article
em En
| MEDLINE
| ID: mdl-2027132
ABSTRACT
Measurement of mRNA in adult articular cartilage could define whether treatment with nonsteroidal antiinflammatory drugs affects the rate of proteoglycan synthesis in vivo in osteoarthritis. We studied a variety of extraction and isolation methods to obtain a high yield of pure mRNA from adult articular cartilage. By combining a single step extraction procedure, precipitation in 0.8 M LiCl and poly d-T affinity isolation, we obtained pure mRNA that hybridized with a cDNA probe for the large aggregating proteoglycan. Thus, it should be feasible to assess the level of message using polymerase chain reaction technology; it is unlikely that northern blots, slot blots, or RNA protection assays can be performed directly on the small specimens of articular cartilage that are available from experimental model studies or from surgical specimens.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteoglicanas
/
RNA Mensageiro
/
Cartilagem Articular
Limite:
Animals
Idioma:
En
Revista:
J Rheumatol Suppl
Ano de publicação:
1991
Tipo de documento:
Article
País de afiliação:
Canadá