Electron microscope study of the base sequence homology between simian virus 40 and human papovavirus BK.
J Virol
; 25(1): 193-201, 1978 Jan.
Article
em En
| MEDLINE
| ID: mdl-202735
ABSTRACT
The base sequence homology between the genomes of simian virus 40 (SV40) and human papovavirus BK (BKV) was studied by the heteroduplex method of Ferguson and Davis (J. Mol. Biol. 94135-149, 1975). When mounted for microscopy in 30% formamide (Tm-35 degrees C), BKV/SV40 heteroduplexes were an average of 92% double-stranded and contained only two small nonhomologous regions that mapped near the junctions between the early and late regions of the SV40 Genome. At higher formamide concentrations, the fraction of duplex DNA in the BKV/SV40 heteroduplexes decreased, indicating significant base mismatching in the homologous regions. The strongest regions of homology were located in the late region.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA Viral
/
Polyomavirus
/
Vírus BK
/
Vírus 40 dos Símios
/
Conformação de Ácido Nucleico
Idioma:
En
Revista:
J Virol
Ano de publicação:
1978
Tipo de documento:
Article