Intestinal lamina propria TcRgammadelta+ lymphocytes selectively express IL-10 and IL-17.
J Investig Allergol Clin Immunol
; 20(5): 391-401, 2010.
Article
em En
| MEDLINE
| ID: mdl-20945605
ABSTRACT
BACKGROUND:
The characteristics and roles of gut lymphocytes have been only partly elucidated, in particular with regard to activation patterns.OBJECTIVES:
To characterize lymphocytes from various parts of the gut and examine their activation pattern as a network.METHODS:
Lymphocytes were isolated from the epithelium, the lamina propria, Peyer's patches, mesenteric lymph nodes, the spleen, and peripheral blood of naive mice. They were then characterized for T cell phenotype, T cell receptors (TcRs), activation markers, and cytokine production.RESULTS:
The results showed a gradient of cells with an increasing proportion of TcRgammadelta+, CD8alphaalpha+ cells towards the gut lumen, with the highest number found in intraepithelial lymphocytes. These cells, together with lamina propria lymphocytes (LPLs) were also characterized by a memory-like phenotype (CD25- CD45RB(low) and CD44(high)) and CD69 expression. CD8+ TcRgammadelta+ LPLs produced IL-10 and IL-17, while TcRalphabeta+ LPLs were FoxP3 positive.CONCLUSIONS:
Gut lymphocytes express various receptors and cytokines according to their location. These specific features suggest a differential function for gut lymphocytes depending on their location.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Linfócitos T
/
Receptores de Antígenos de Linfócitos T gama-delta
/
Interleucina-10
/
Interleucina-17
/
Mucosa Intestinal
Limite:
Animals
Idioma:
En
Revista:
J Investig Allergol Clin Immunol
Assunto da revista:
ALERGIA E IMUNOLOGIA
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Suíça