Colon extended physiologically based extraction test (CE-PBET) increases bioaccessibility of soil-bound PAH.
Environ Sci Technol
; 45(12): 5301-8, 2011 Jun 15.
Article
em En
| MEDLINE
| ID: mdl-21568264
ABSTRACT
Assessment of the risk to human health posed by contaminated land may be seriously overestimated if reliant on total pollutant concentration. In vitro extraction tests, such as the physiologically based extraction test (PBET), imitate the physicochemical conditions of the human gastro-intestinal tract and offer a more practicable alternative for routine testing purposes. However, even though passage through the colon accounts for approximately 80% of the transit time through the human digestive tract and the typical contents of the colon in vivo are a carbohydrate-rich aqueous medium with the potential to promote desorption of organic pollutants, PBET comprises stomach and small intestine compartments only. Through addition of an eight-hour colon compartment to PBET and use of a carbohydrate-rich fed-state medium we demonstrated that colon-extended PBET (CE-PBET) increased assessments of soil-bound PAH bioaccessibility by up to 50% in laboratory soils and a factor of 4 in field soils. We attribute this increased bioaccessibility to a combination of the additional extraction time and the presence of carbohydrates in the colon compartment, both of which favor PAH desorption from soil. We propose that future assessments of the bioaccessibility of organic pollutants in soils using physiologically based extraction tests should have a colon compartment as in CE-PBET.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Contexto em Saúde:
2_ODS3
Problema de saúde:
2_quimicos_contaminacion
Assunto principal:
Hidrocarbonetos Policíclicos Aromáticos
/
Solo
/
Monitoramento Ambiental
/
Colo
Limite:
Humans
Idioma:
En
Revista:
Environ Sci Technol
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Reino Unido