Stress-induced expression of the transcription factor RERJ1 is tightly regulated in response to jasmonic acid accumulation in rice.

ABSTRACT

The plant hormone jasmonic acid (JA) regulates various developmental processes and plant defence responses to environmental stresses. We previously reported that RERJ1, a JA-inducible transcription factor in rice, is up-regulated by exposure to wounding and drought stress. Here, we demonstrated that the expression of RERJ1 after wounding is regulated in a JA-dependent manner in rice, based on histochemical analysis of RERJ1 promoter-GUS transgenic plants. RERJ1 expression was induced only at the region of injury after wounding, whereas expression was induced in the entire leaf after drought. According to JA measurements of stressed leaves, high accumulation of endogenous JA was only detected around the wound site in a rice leaves, whereas the drought treatment led to uniform accumulation of JA in the entire leaf, suggesting that RERJ1 will be a useful marker gene for studies on localization of JA in rice. Nuclear localization and transactivation ability of RERJ1 were also demonstrated. These results suggest that RERJ1 plays a role as a transcriptional activator for regulating stress-inducible gene expression, with a strong correlation to JA accumulation in the stressed region.