Intravital microscopy to study myocardial engraftment.
Interact Cardiovasc Thorac Surg
; 15(1): 5-9, 2012 Jul.
Article
em En
| MEDLINE
| ID: mdl-22457185
ABSTRACT
Tissue engineering and stem cell transplantation are promising novel therapies for myocardial repair. A major barrier to cell survival after transplantation involves inadequate vascularization. Continuous observation of cardiac tissue engraftment and angiogenesis could help understand these processes and allow for identification of the optimal conditions for these therapeutic interventions. We investigated the ability of a skin-fold chamber model to allow for engraftment of differentiated myocardial tissue in mice. Neonatal atrial and ventricular tissues were implanted in the in vivo chambers. All myocardial implants had a high rate of engraftment (86-95%). Tissue engraftment was preceded by a 'bleeding phase' in both the atrial and ventricular implants. This occurred earlier in ventricular compared with atrial implants. Spontaneous contractions were observed after an average of 13 days after implantation in all chambers but occurred earlier in ventricular compared with atrial implants. The host cells surrounded the myocardial implants circumferentially, but have limited infiltration into these grafts. This is the first report of successful ectopic engraftment of differentiated myocardium using a skin-fold chamber. This model is invaluable for real-time observation of early angiogenesis and tissue growth during in vivo myocardial engineering and myocardial regeneration.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Regeneração
/
Transplante de Coração
/
Microscopia de Vídeo
/
Neovascularização Fisiológica
/
Sobrevivência de Enxerto
/
Microscopia de Fluorescência
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Interact Cardiovasc Thorac Surg
Assunto da revista:
ANGIOLOGIA
/
CARDIOLOGIA
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Estados Unidos