[Nicotine regulates large conductance ca2+ activated K+ channels in rat coronary arterial smooth muscle cells].
Zhongguo Ying Yong Sheng Li Xue Za Zhi
; 28(1): 24-7, 2012 Jan.
Article
em Zh
| MEDLINE
| ID: mdl-22493888
ABSTRACT
OBJECTIVE:
The present study was to explore signaling mechanisms underlying nicotine-induced inhibition of large-conductance calcium-activated potassium channels (BK(Ca)).METHODS:
8 week male Wistar rats were divided randomly into saline group and nicotine group and received respectively injection with saline or nicotine (Sigma, Shanghai, China) at 2 mg/(kg x d) for 21 days. Coronary vascular smooth muscle cells were dissociated enzymatically. Dissociated smooth muscle cells were interfered with CPT-cAMP (100 micromol/L) or forskolin (10 micromol/L). The signal channel open dwell-time (To), close dwell-time (Tc) and open probability (Po) were recorded.RESULTS:
CPT-cAMP or forskolin significantly prolonged To, shorten Tc and increased Po in saline group (P < 0.01). But in nicotine group To, Tc and Po did not been changed.CONCLUSION:
This phenomenon may serve as a physiological mechanism that nicotine inhibits BK(Ca) channel activity to increase via cAMP/PKA-dependent pathway.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vasos Coronários
/
Miócitos de Músculo Liso
/
Canais de Potássio Ativados por Cálcio de Condutância Alta
/
Nicotina
Limite:
Animals
Idioma:
Zh
Revista:
Zhongguo Ying Yong Sheng Li Xue Za Zhi
Assunto da revista:
FISIOLOGIA
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
China