Immunostimulatory Effects of ß-glucan Purified from Paenibacillus polymyxa JB115 on Mouse Splenocytes.
Korean J Physiol Pharmacol
; 16(4): 225-30, 2012 Aug.
Article
em En
| MEDLINE
| ID: mdl-22915986
ABSTRACT
We investigated the effects of ß-glucan purified from Paenibacillus polymyxa JB115 on the viability and proliferation of splenocytes. Splenocytes play a critical role in host immunity. MTT assays and trypan blue exclusion tests revealed that ß-glucan significantly promoted the viability and proliferation of splenocytes over a range of concentrations. However, there was no specific subset change. ß-glucan protected splenocytes from cytokine withdrawal-induced spontaneous cell death. For further mechanistic studies, ELISA assay revealed that ß-glucan enhanced the expression of anti-apoptotic molecules and interleukin 7 (IL-7), a cytokine critical for lymphocyte survival. We also investigated the IL-2 dependency of ß-glucan-treated splenocytes to determine if treated cells could still undergo clonal expansion. In flow cytometric analysis, ß-glucan induced increased levels of the activation marker CD25 on the surface of splenocytes and ß-glucan-treated splenocytes showed higher proliferation rates in response to IL-2 treatment. This study demonstrates that ß-glucan can enhance the survival of splenocytes and provides valuable information to broaden the use of ß-glucan in research fields.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Korean J Physiol Pharmacol
Ano de publicação:
2012
Tipo de documento:
Article