Screening natural antioxidants in peanut shell using DPPH-HPLC-DAD-TOF/MS methods.
Food Chem
; 135(4): 2366-71, 2012 Dec 15.
Article
em En
| MEDLINE
| ID: mdl-22980814
ABSTRACT
Peanut shell, a byproduct in oil production, is rich in natural antioxidants. Here, a rapid and efficient method using DPPH-HPLC-DAD-TOF/MS was used for the first time to screen antioxidants in peanut shell. The method is based on the hypothesis that upon reaction with 1, 1-diphenyl-2-picrylhydrazyl (DPPH), the peak areas of compounds with potential antioxidant activities in the HPLC chromatogram will be significantly reduced or disappeared, and the identity confirmation could be achieved by HPLC-DAD-TOF/MS technique. With this method, three compounds possessing potential antioxidant activities were found abundantly in the methanolic extract of peanut shell. They were identified as 5,7-dihydroxychromone, eriodictyol, and luteolin. The contents of these compounds were 0.59, 0.92, and 2.36 mg/g, respectively, and luteolin possessed the strongest radical scavenging capacity. DPPH-HPLC-DAD-TOF/MS assay facilitated rapid identification and determination of natural antioxidants in peanut shell, which may be helpful for value-added utilization of peanut processing byproducts.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Arachis
/
Espectrometria de Massas
/
Extratos Vegetais
/
Cromatografia Líquida de Alta Pressão
/
Antioxidantes
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
/
Screening_studies
Idioma:
En
Revista:
Food Chem
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
China