Voluntary ban on cephalosporin use in Danish pig production has effectively reduced extended-spectrum cephalosporinase-producing Escherichia coli in slaughter pigs.
J Antimicrob Chemother
; 68(3): 569-72, 2013 Mar.
Article
em En
| MEDLINE
| ID: mdl-23129728
ABSTRACT
OBJECTIVES:
To measure the effect of a voluntary ban on cephalosporin usage in the Danish pig production on the prevalence of extended-spectrum cephalosporinase (ESC)-producing Escherichia coli in pigs and pork.METHODS:
Data on cephalosporin consumption were obtained from the VetStat database. For detection of ESC-producing E. coli, three sampling types were included at slaughter, caecal samples were collected from pigs in 2009 and 2010 (June) before and in two periods (2010 and 2011) after a voluntary ban on cephalosporins was effected (July 2010); at farm level, pools of five stool samples from different pigsties were collected in 2010 and in 2011; and samples from pork were collected randomly at retail stores and outlets from 2009 to 2011. ESC-producing E. coli was isolated after selective enrichment in MacConkey broth with 1 mg/L ceftriaxone. ESC genes were detected using PCR, microtube array and sequencing.RESULTS:
From July 2010 the consumption of cephalosporins approximated zero. The occurrence of ESC-producing E. coli in pigs at slaughter was not significantly different (P=0.7) between 2009 [10.8% (85/786)] and 2010 [11.8% (48/407)], but in 2011 the occurrence [3.6% (28/777)] decreased significantly (P<0.001). A significant decrease (P=0.002) in occurrence of ESC-producing E. coli at pig farm level from 2010 [11% (11/99)] to 2011 (0/78) was also observed. The bla(CTX-M-1) gene was most often detected (63%), but bla(CTX-M-14) and bla(CTX-M-15) were also found. Occurrence in pork was between 1.3% and 0.9%.CONCLUSIONS:
The discontinuation of an already low use of cephalosporins in pig production has significantly reduced the occurrence of ESC-producing E. coli.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Contexto em Saúde:
2_ODS3
/
3_ND
Problema de saúde:
2_enfermedades_transmissibles
/
3_neglected_diseases
/
3_zoonosis
Assunto principal:
Cefalosporinase
/
Cefalosporinas
/
Dieta
/
Escherichia coli
/
Infecções por Escherichia coli
/
Criação de Animais Domésticos
/
Antibacterianos
Tipo de estudo:
Prevalence_studies
/
Risk_factors_studies
Limite:
Animals
País/Região como assunto:
Europa
Idioma:
En
Revista:
J Antimicrob Chemother
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Dinamarca