Long term cryopreservation in 5% DMSO maintains unchanged CD34(+) cells viability and allows satisfactory hematological engraftment after peripheral blood stem cell transplantation.
Vox Sang
; 105(1): 77-80, 2013 Jul.
Article
em En
| MEDLINE
| ID: mdl-23384290
ABSTRACT
Peripheral blood stem cell cryopreservation is associated with cell damage and decreased viability. We evaluated the impact of up to 10 years of cryopreservation (5% DMSO) on viability of CD34(+) cells utilizing graft samples of consecutive patients (2002-2012) with different malignancies who underwent stem cell collection and transplantation. Viability of CD34(+) cells from oncohaematological patients measured after 5 weeks (97·2 ± 0·6%) or after 9-10 years of cryopreservation (95·9 ± 0·5%) was unaffected. Haemoglobin, granulocyte and platelet recovery after transplantation of long-term cryopreserved grafts occurred within 8-13 days. CD34(+) stem cells can be safely stored up to 9-10 years, without affecting cell viability and clinical effectiveness.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células-Tronco Hematopoéticas
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Criopreservação
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Dimetil Sulfóxido
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Crioprotetores
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Transplante de Células-Tronco de Sangue Periférico
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Sobrevivência de Enxerto
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Neoplasias
Limite:
Female
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Humans
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Male
Idioma:
En
Revista:
Vox Sang
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Itália