Identification of a subpopulation of nasopharyngeal carcinoma cells with cancer stem-like cell properties by high aldehyde dehydrogenase activity.
Laryngoscope
; 123(8): 1903-11, 2013 Aug.
Article
em En
| MEDLINE
| ID: mdl-23616401
ABSTRACT
OBJECTIVES/HYPOTHESIS:
Cancer stem cells have been reported as a new therapeutic target in many cancers, but their existence in nasopharyngeal carcinoma (NPC) is largely unknown. This study was conducted to determine cancer stem-like cells in NPC cell line. STUDYDESIGN:
Basic science experimental study.METHODS:
Aldehyde dehydrogenase (ALDH) activity, a putative functional marker for cancer stem cells, was assessed in Epstein-Barr virus-associated NPC cell line C666-1 cells. The ability of cells with high and low ALDH activity to proliferate, resist therapy, and initiate tumor formation was compared.RESULTS:
Enrichment of cancer stem-like cells (with high ALDH activity) in C666-1 was associated with a significantly greater ability to proliferate, be clonogenic, resist chemotherapy drugs and radiation, reconstitute a heterogeneous population, and express pluripotent markers. Furthermore, subcutaneous injection of these cells into immunodeficient nude mice resulted in a tendency of tumor formation at a higher rate as compared to cells with low ALDH activity.CONCLUSIONS:
These results provide evidence for the existence of cancer stem-like cells in the NPC cell line C666-1 cells.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células-Tronco Neoplásicas
/
Biomarcadores Tumorais
/
Separação Celular
/
Neoplasias Nasofaríngeas
/
Herpesvirus Humano 4
/
Aldeído Desidrogenase
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Laryngoscope
Assunto da revista:
OTORRINOLARINGOLOGIA
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Singapura