A eukaryotic molecular target candidate of roxithromycin: fungal differentiation as a sensitive drug target analysis system.
Biosci Biotechnol Biochem
; 77(7): 1539-47, 2013.
Article
em En
| MEDLINE
| ID: mdl-23832352
ABSTRACT
Roxithromycin (RXM), active against prokaryotes, has beneficial side effects such as anti-cancer activities on mammalian cells, but the mechanisms underlying these effects remain unclear. We found that RXM inhibited the cellular differentiation of the rice blast fungus Magnaporthe oryzae. Hence, we screened the targets of RXM by the T7 phage display method with fungal genomic DNA, and identified MoCDC27 (M. oryzae Cell Division Cycle 27) as a candidate. We generated mocdc27 knockdown mutants that the appressoria formation was less affected by RXM. A complemented mutant restored sensitivity against RXM to the level of the wild type. These results suggest that MoCDC27 was involved in the inhibition of appressorium formation by RXM, and that the complex of RXM-MoCDC27 affected another molecule involved in appressorium formation. The T7 phage display method with fungal genomic DNA can be a useful tool in the quest for drug target.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Roxitromicina
/
Magnaporthe
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Biosci Biotechnol Biochem
Assunto da revista:
BIOQUIMICA
/
BIOTECNOLOGIA
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Japão