Viability, longevity, and egg production of Drosophila melanogaster are regulated by the miR-282 microRNA.
Genetics
; 195(2): 469-80, 2013 Oct.
Article
em En
| MEDLINE
| ID: mdl-23852386
ABSTRACT
The first microRNAs were discovered some 20 years ago, but only a small fraction of the microRNA-encoding genes have been described in detail yet. Here we report the molecular analysis of a computationally predicted Drosophila melanogaster microRNA gene, mir-282. We show that the mir-282 gene is the source of a 4.9-kb-long primary transcript with a 5' cap and a 3'-poly(A) sequence and a mature microRNA of â¼25 bp. Our data strongly suggest the existence of an independent mir-282 gene conserved in holometabolic insects. We give evidence that the mir-282 locus encodes a functional transcript that influences viability, longevity, and egg production in Drosophila. We identify the nervous system-specific adenylate cyclase (rutabaga) as a target of miR-282 and assume that one of the main functions of mir-282 is the regulation of adenylate cyclase activity in the nervous system during metamorphosis.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Adenilil Ciclases
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MicroRNAs
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Drosophila melanogaster
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Longevidade
Limite:
Animals
Idioma:
En
Revista:
Genetics
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Hungria