Increased γ-H2AX by exposure to a 60-Hz magnetic fields combined with ionizing radiation, but not hydrogen peroxide, in non-tumorigenic human cell lines.
Int J Radiat Biol
; 90(4): 291-8, 2014 Apr.
Article
em En
| MEDLINE
| ID: mdl-24467330
ABSTRACT
PURPOSE:
Genotoxic effects have been considered the gold standard to determine if an environmental factor is a carcinogen, but the currently available data for extremely low frequency time-varying magnetic fields (ELF-MF) remain controversial. As an environmental stimulus, the effect of ELF-MF on cellular DNA may be subtle. Therefore, a more sensitive method and systematic research strategy are warranted to evaluate genotoxicity. MATERIALS ANDMETHODS:
We investigated the effect of ELF-MF in combination with ionizing radiation (IR) or H(2)O(2) on the DNA damage response of expression of phosphorylated H2AX (γ-H2AX) and production of γ-H2AX foci in non-tumorigenic human cell systems consisting of human lung fibroblast WI-38 cells and human lung epithelial L132 cells.RESULTS:
Exposure to a 60-Hz, 2 mT ELF-MF for 6 h produced increased γ-H2AX expression, as well as γ-H2AX foci production, a common DNA double-strand break (DSB) marker. However, exposure to a 1 mT ELF-MF did not have the same effect. Moreover, 2 mT ELF-MF exposure potentiated the expression of γ-H2AX and γ-H2AX foci production when combined with IR, but not when combined with H(2)O(2).CONCLUSIONS:
ELF-MF could affect the DNA damage response and, in combination with different stimuli, provide different effects on γ-H2AX.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Radiação Ionizante
/
Histonas
/
Campos Magnéticos
/
Peróxido de Hidrogênio
Limite:
Humans
Idioma:
En
Revista:
Int J Radiat Biol
Assunto da revista:
RADIOLOGIA
Ano de publicação:
2014
Tipo de documento:
Article