Multi-isotype antibody responses against the multimeric Salmonella Typhi recombinant hemolysin E antigen.
Microbiol Immunol
; 59(1): 43-7, 2015 Jan.
Article
em En
| MEDLINE
| ID: mdl-25399538
ABSTRACT
The detection and measurement of different antibody isotypes in the serum provide valuable indicators of the different stages of typhoid infection. Here, the ability of S. Typhi recombinant hemolysin E (HlyE) to detect multi-isotype antibody responses in sera of patients with typhoid and paratyphoid A was investigated using an indirect antibody immunoassay. Nanogram amounts of HlyE were found to be sufficient for detection of IgG and IgA isotypes and, in a study of individuals' sera (n = 100), the immunoassay was able to distinguish between typhoid and non-typhoid sera. The overall sensitivity, specificity and efficiency of the ELISA were 70% (39/56), 100% (44/44) and 83% respectively.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Contexto em Saúde:
3_ND
Problema de saúde:
3_neglected_diseases
/
3_zoonosis
Assunto principal:
Salmonella typhi
/
Febre Tifoide
/
Testes Diagnósticos de Rotina
/
Proteínas Hemolisinas
/
Anticorpos Antibacterianos
/
Antígenos de Bactérias
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Limite:
Humans
Idioma:
En
Revista:
Microbiol Immunol
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Malásia