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APP intracellular domain acts as a transcriptional regulator of miR-663 suppressing neuronal differentiation.
Shu, R; Wong, W; Ma, Q H; Yang, Z Z; Zhu, H; Liu, F J; Wang, P; Ma, J; Yan, S; Polo, J M; Bernard, C C A; Stanton, L W; Dawe, G S; Xiao, Z C.
Afiliação
  • Shu R; 1] Shunxi-Monash Immune Regeneration and Neuroscience Laboratories, Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria 3800, Australia [2] The Key Laboratory of Stem Cell and Regenerative Medicine, Institute of Molecular and Clinical Medicine, Kunming Medical Colle
  • Wong W; 1] Department of Pharmacology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore, Singapore 119077, Singapore [2] Neurobiology and Ageing Programme, Life Sciences Institute, National University of Singapore, Singapore 119077, Singapore [3] Singapore
  • Ma QH; Institute of Neuroscience, Soochow University, Suzhou 215123, China.
  • Yang ZZ; 1] Shunxi-Monash Immune Regeneration and Neuroscience Laboratories, Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria 3800, Australia [2] The Key Laboratory of Stem Cell and Regenerative Medicine, Institute of Molecular and Clinical Medicine, Kunming Medical Colle
  • Zhu H; 1] Shunxi-Monash Immune Regeneration and Neuroscience Laboratories, Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria 3800, Australia [2] The Key Laboratory of Stem Cell and Regenerative Medicine, Institute of Molecular and Clinical Medicine, Kunming Medical Colle
  • Liu FJ; 1] Australian Regenerative Medicine Institute, Monash University, Clayton, Victoria 3800, Australia [2] College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471000, China.
  • Wang P; Department of Pediatrics, Duke University School of Medicine, Durham, NC 27710, USA.
  • Ma J; Shunxi-Monash Immune Regeneration and Neuroscience Laboratories, Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria 3800, Australia.
  • Yan S; The Key Laboratory of Stem Cell and Regenerative Medicine, Institute of Molecular and Clinical Medicine, Kunming Medical College, Kunming 650031, China.
  • Polo JM; 1] Shunxi-Monash Immune Regeneration and Neuroscience Laboratories, Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria 3800, Australia [2] Australian Regenerative Medicine Institute, Monash University, Clayton, Victoria 3800, Australia.
  • Bernard CC; Australian Regenerative Medicine Institute, Monash University, Clayton, Victoria 3800, Australia.
  • Stanton LW; Stem Cell and Developmental Biology Group, Genome Institute of Singapore, Singapore 138672, Singapore.
  • Dawe GS; 1] Department of Pharmacology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore, Singapore 119077, Singapore [2] Neurobiology and Ageing Programme, Life Sciences Institute, National University of Singapore, Singapore 119077, Singapore [3] Singapore
  • Xiao ZC; 1] Shunxi-Monash Immune Regeneration and Neuroscience Laboratories, Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria 3800, Australia [2] The Key Laboratory of Stem Cell and Regenerative Medicine, Institute of Molecular and Clinical Medicine, Kunming Medical Colle
Cell Death Dis ; 6: e1651, 2015 Feb 19.
Article em En | MEDLINE | ID: mdl-25695604
ABSTRACT
Amyloid precursor protein (APP) is best known for its involvement in the pathogenesis of Alzheimer's disease. We have previously demonstrated that APP intracellular domain (AICD) regulates neurogenesis; however, the mechanisms underlying AICD-mediated regulation of neuronal differentiation are not yet fully characterized. Using genome-wide chromatin immunoprecipitation approaches, we found that AICD is specifically recruited to the regulatory regions of several microRNA genes, and acts as a transcriptional regulator for miR-663, miR-3648 and miR-3687 in human neural stem cells. Functional assays show that AICD negatively modulates neuronal differentiation through miR-663, a primate-specific microRNA. Microarray data further demonstrate that miR-663 suppresses the expression of multiple genes implicated in neurogenesis, including FBXL18 and CDK6. Our results indicate that AICD has a novel role in suppression of neuronal differentiation via transcriptional regulation of miR-663 in human neural stem cells.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Diferenciação Celular / Precursor de Proteína beta-Amiloide / MicroRNAs / Neurônios Limite: Humans Idioma: En Revista: Cell Death Dis Ano de publicação: 2015 Tipo de documento: Article
Texto completo
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XML
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Diferenciação Celular / Precursor de Proteína beta-Amiloide / MicroRNAs / Neurônios Limite: Humans Idioma: En Revista: Cell Death Dis Ano de publicação: 2015 Tipo de documento: Article