Osteogenic potential of mesenchymal cells derived from canine umbilical cord matrix co-cultured with platelet-rich plasma and demineralized bone matrix.
J Vet Sci
; 16(3): 381-4, 2015.
Article
em En
| MEDLINE
| ID: mdl-26040617
ABSTRACT
Canine mesenchymal cells (MSCs) derived from Wharton's jelly were co-cultured, then supplemented or not supplemented with platelet rich plasma (PRP) and demineralized bone matrix (DBM) to verify osteogenic differentiation. Osteoblastic differentiation followed by mineralized bone matrix production was found to be significantly higher (p < 0.05) when MSCs were associated with PRP/DBM in culture after 14-21-days of induction. Osteopontin and osteocalcin gene expression were significantly superior (p < 0.05) under the same culture conditions after 21 days of observation. In conclusion, addition of PRP to DBM co-cultured with MSCs successfully induced osteogenesis in vitro.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Osteogênese
/
Matriz Óssea
/
Plasma Rico em Plaquetas
/
Células-Tronco Mesenquimais
Limite:
Animals
Idioma:
En
Revista:
J Vet Sci
Assunto da revista:
MEDICINA VETERINARIA
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Brasil