Tyrosinase inhibition kinetic studies of standardized extract of Berberis aristata.
Nat Prod Res
; 30(12): 1451-4, 2016 Jun.
Article
em En
| MEDLINE
| ID: mdl-26212353
ABSTRACT
The stem bark and wood of Berberis aristata DC (Daruharidra) are one of the principal ingredients of traditional skin lighting and exfoliating scrub preparation in India. The standardised extract of B. aristata was screened to evaluate their in vitro antityrosinase activity and inhibition kinetics. Phytochemical and pharmacological studies were carried out with different solvent fractions of the methanol extract of B. aristata (MEBA). RP-HPLC analysis was used to determine the berberine content in extract and fractions of B. aristata. MEBA showed maximum berberine content. Extract and fractions of B. aristata contain the maximum amount of alkaloids than other constituents. In tyrosinase inhibition assay, MEBA was found to possess highest dose-dependent monophenolase and moderate diphenolase activity. The enzyme kinetic study revealed that MEBA possessed mixed type inhibition of monophenolase activity of tyrosinase. These bioactivities indicate that the MEBA has antihyperpigmentation potential in human skin.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Extratos Vegetais
/
Monofenol Mono-Oxigenase
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Berberis
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Inibidores Enzimáticos
País/Região como assunto:
Asia
Idioma:
En
Revista:
Nat Prod Res
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Índia