Detection of cellular prion protein in exosomes derived from ovine plasma.
J Gen Virol
; 96(12): 3698-3702, 2015 Dec.
Article
em En
| MEDLINE
| ID: mdl-26399471
ABSTRACT
Prion protein (PrP) is present at extremely low levels in the blood of animals and its detection is complicated by the poor sensitivity of current standard methodologies. Interesting results have been obtained with recent advanced technologies that are able to detect minute amounts of the pathological PrP (PrPSc), but their efficiency is reduced by various factors present in blood. In this study, we were able to extract cellular PrP (PrPC) from plasma-derived exosomes by a simple, fast method without the use of differential ultracentrifugation and to visualize it by Western blotting, reducing the presence of most plasma proteins. This result confirms that blood is capable of releasing PrP in association with exosomes and could be useful to better study its role in the pathogenesis of transmissible spongiform encephalopathies.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Scrapie
/
Príons
/
Exossomos
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
J Gen Virol
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Itália