Three-Dimensional Neural Spheroid Culture: An In Vitro Model for Cortical Studies.
Tissue Eng Part C Methods
; 21(12): 1274-83, 2015 Dec.
Article
em En
| MEDLINE
| ID: mdl-26414693
ABSTRACT
There is a high demand for in vitro models of the central nervous system (CNS) to study neurological disorders, injuries, toxicity, and drug efficacy. Three-dimensional (3D) in vitro models can bridge the gap between traditional two-dimensional culture and animal models because they present an in vivo-like microenvironment in a tailorable experimental platform. Within the expanding variety of sophisticated 3D cultures, scaffold-free, self-assembled spheroid culture avoids the introduction of foreign materials and preserves the native cell populations and extracellular matrix types. In this study, we generated 3D spheroids with primary postnatal rat cortical cells using an accessible, size-controlled, reproducible, and cost-effective method. Neurons and glia formed laminin-containing 3D networks within the spheroids. The neurons were electrically active and formed circuitry through both excitatory and inhibitory synapses. The mechanical properties of the spheroids were in the range of brain tissue. These in vivo-like features of 3D cortical spheroids provide the potential for relevant and translatable investigations of the CNS in vitro.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Esferoides Celulares
/
Técnicas de Cultura de Células
/
Microambiente Celular
/
Neurônios
Limite:
Animals
Idioma:
En
Revista:
Tissue Eng Part C Methods
Assunto da revista:
BIOTECNOLOGIA
/
HISTOLOGIA
Ano de publicação:
2015
Tipo de documento:
Article