Buspirone requires the intact nigrostriatal pathway to reduce the activity of the subthalamic nucleus via 5-HT1A receptors.
Exp Neurol
; 277: 35-45, 2016 Mar.
Article
em En
| MEDLINE
| ID: mdl-26687972
ABSTRACT
The most effective treatment for Parkinson's disease (PD), l-DOPA, induces dyskinesia after prolonged use. We have previously shown that in 6-hydroxydopamine (6-OHDA) lesioned rats rendered dyskinetic by prolonged l-DOPA administration, lesion of the subthalamic nucleus (STN) reduces not only dyskinesias but also buspirone antidyskinetic effect. This study examined the effect of buspirone on STN neuron activity. Cell-attached recordings in parasagittal slices from naïve rats showed that whilst serotonin excited the majority of STN neurons, buspirone showed an inhibitory main effect but only in 27% of the studied cells which was prevented by the 5-HT1A receptor selective antagonist WAY-100635. Conversely, single-unit extracellular recordings were performed in vivo on STN neurons from four different groups, i.e., control, chronically treated with l-DOPA, 6-OHDA lesioned and lesioned treated with l-DOPA (dyskinetic) rats. In control animals, systemic-buspirone administration decreased the firing rate in a dose-dependent manner in every cell studied. This effect, prevented by WAY-100635, was absent in 6-OHDA lesioned rats and was not modified by prolonged l-DOPA administration. Altogether, buspirone in vivo reduces consistently the firing rate of the STN neurons through 5-HT1A receptors whereas ex vivo buspirone seems to affect only a small population of STN neurons. Furthermore, the lack of effect of buspirone in 6-OHDA lesioned rats, suggests the requirement of not only the activation of 5-HT1A receptors but also an intact nigrostriatal pathway for buspirone to inhibit the STN activity.
Palavras-chave
6-Hydroxydopamine (PubChem CID: 4624); 6-OHDA lesion; Basal ganglia; Buspirone hydrochloride (PubChem CID: 36431); D-AP5 (PubChem CID: 135342); DNQX (PubChem CID: 3899541); Dyskinesia; Gabazine (SR95531 hydrobromide, PubChem CID: 107895); Parkinson's disease; Serotonin; Serotonin hydrochloride (PubChem CID: 160436); WAY-100635 maleate salt (PubChem CID: 11957721); l-DOPA; l-DOPA (PubChem CID: 6047)
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Contexto em Saúde:
1_ASSA2030
Problema de saúde:
1_doencas_nao_transmissiveis
Assunto principal:
Buspirona
/
Agonistas do Receptor de Serotonina
/
Núcleo Subtalâmico
/
Receptor 5-HT1A de Serotonina
/
Neurônios
Tipo de estudo:
Etiology_studies
Limite:
Animals
Idioma:
En
Revista:
Exp Neurol
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Espanha