Metabolite Profiles of Icariin in Rat Feces, Bile and Urine by Ultraperformance Liquid-Chromatography/Quadrupole-Time-of-Flight Mass Spectrometry.
J Chromatogr Sci
; 54(2): 158-64, 2016 Feb.
Article
em En
| MEDLINE
| ID: mdl-26792083
ABSTRACT
In this work, the metabolite profiles of icariin in rat feces, bile and urine were qualitatively investigated, and the possible metabolic pathways of icariin were subsequently proposed. After oral administration of icariin at a single dose of 100 mg/kg, rat biological samples were collected and pretreated. Then, these pretreated samples were detected by ultraperformance liquid chromatography/quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF-MS). In all, 17 metabolites were identified in the biosamples. Of these, 5, including F8-F9 (icariside I), D3-D4 (isopentenyl alcohol-icaritin-3-O-rha-7-O-gluA) and N3 (1,3-isoprene icariside II), were to our knowledge reported for the first time. The results indicated that icariin was metabolized via desugarization, dehydrogenation, hydroxylation, demethylation and glucuronidation pathways in vivo. This study revealed the possible metabolite profiles of icariin in rats.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bile
/
Flavonoides
/
Medicamentos de Ervas Chinesas
/
Cromatografia Líquida de Alta Pressão
/
Epimedium
/
Espectrometria de Massas em Tandem
/
Fezes
Tipo de estudo:
Evaluation_studies
Limite:
Animals
Idioma:
En
Revista:
J Chromatogr Sci
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
China