Characterization of human adipose tissue-derived stem cells with enhanced angiogenic and adipogenic properties.
J Tissue Eng Regen Med
; 11(9): 2490-2502, 2017 09.
Article
em En
| MEDLINE
| ID: mdl-26833948
ABSTRACT
Autologous fat grafting is a popular method for soft tissue reconstructions but graft survival remains highly unpredictable. Supplementation of the graft with the stromal vascular fraction (SVF) or cultured adipose tissue-derived stem cells (ASCs) can enhance graft viability. In this study we have examined the phenotypic properties of a selected population of cells isolated from ASCs, with a view to determining their suitability for transplantation into grafts. ASCs were isolated from the SVF of human abdominal fat (n = 8 female patients) and CD146+ cells were selected using immunomagnetic beads. The angiogenic and adipogenic properties of the positively selected cells were compared with the negative fraction. CD146+ cells expressed the immunophenotypic characteristics of pericytes. With prolonged in vitro expansion, CD146- cells exhibited increased population doubling times and morphological signs of senescence, whereas CD146+ cells did not. CD146+ cells expressed higher levels of the angiogenic molecules VEGF-A, angiopoietin-1 and FGF-1. Conditioned medium taken from CD146+ cells significantly increased formation of in vitro endothelial cell tube networks, whereas CD146- cells did not. CD146+ cells could be differentiated into adipocytes in greater numbers than CD146- cells. Consistent with this, differentiated CD146+ cells expressed higher levels of the adipocyte markers adiponectin and leptin. These results suggest that CD146+ cells selected from a heterogeneous mix of ASCs have more favourable angiogenic and adipogenic properties, which might provide significant benefits for reconstructive and tissue-engineering applications. Copyright © 2016 John Wiley & Sons, Ltd.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células-Tronco
/
Diferenciação Celular
/
Tecido Adiposo
/
Neovascularização Fisiológica
Limite:
Humans
Idioma:
En
Revista:
J Tissue Eng Regen Med
Assunto da revista:
BIOTECNOLOGIA
/
HISTOLOGIA
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Suécia